A report on the interlaboratory quantitation of haemoglobin A2 and haemoglobin F

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A report on the interlaboratory quantitation of haemoglobin A2 and haemoglobin F.

An interlaboratory trial of the quantitation of Hb A(2) and Hb F has been carried out on two samples of blood by 90 laboratories in the British Isles. The results have been compared with those obtained by four reference laboratories. Overall, the correlation was very poor and indicated that the methods used have to be standardized before interlaboratory values become meaningful. Moreover, in ma...

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Haemoglobin A2 level in pregnancy.

The haemoglobin A(2) level was measured in 217 pregnant and 137 non-pregnant patients. Twenty-eight patients were reinvestigated at least six weeks after delivery. There was no significant difference in the mean haemoglobin A(2) level in pregnancy (2.4 +/- 0.8%) and the mean haemoglobin A(2) level in the non-pregnant controls (2.3 +/- 0.6%). In all anaemic patients whose A(2) level was raised i...

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The properties and reactions of haemoglobin F(1) and their bearing on the dissociation equilibrium of haemoglobin.

1. Hb-F(I), with the previously proposed structure alpha(A) (2)gamma(F)gamma(AcF), would be an exception to the general hypothesis that haemoglobins are in mobile equilibrium with their sub-units at all pH values. However, studies presented in this paper suggest that this is not so. 2. Gel-filtration and sedimentation analyses show that Hb-F(I) dissociates at acid pH and gives only the usual ty...

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Haemoglobin Binding with Haptoglobin UNEQUIVOCAL DEMONSTRATION THAT THE f-CHAINS OF HUMAN HAEMOGLOBIN

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The estimation of haemoglobin A2: is visual assessment reliable?

Haemoglobin A(2) in 11 normal and 42 B thalassaemia minor subjects was estimated by simple visual assessment of the electrophoretogram. The results obtained give rise to doubts as to the reliability of this technique when used to estimate haemoglobin A(2) under normal laboratory conditions.

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1973

ISSN: 0021-9746

DOI: 10.1136/jcp.26.11.864